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2-Plants: Non-GE salt-resistant wheat tested successfully in Australia
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PART I
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TITLE: Salt-tolerant wheats yield double
SOURCE: Countryman, Australia, by Lara Ladyman
http://countryman.thewest.com.au/20040729/cm-home-sto128808.html
DATE: 29 Jul 2004
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Salt-tolerant wheats yield double
GENETICALLY modified salt-tolerant Westonia wheat in glasshouse trials has
produced double the number of seeds of its non-GM counterpart growing in
saline water a third as salty as seawater. Harvested in May this year, the
results provide the first quantitative proof for a WA wheat breeding
company of how its salt tolerant gene is working.
[GENET/HM: with regard to the legal definitions of GE this wheat is not GE
but a cross between common sea barley grass and wheat; according to a
Cooperative Research Centre publication, hybrid embryos had been cultured
on nutrient medium, subsequently applying chromosome doubling and haploid
techniques. In the end a wheat hybrid with an additional barley chromosome
was produced).
PART II
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TITLE: Waterlogging key for saline wheat
SOURCE: Countryman, Australia
http://countryman.thewest.com.au/20040729/cm-home-sto128809.html
DATE: 29 Jul 2004
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Waterlogging key for saline wheat
WORLD'S first research underway in WA is attempting to make wheat more
waterlogging and salt tolerant. The Cooperative Research Centre for Plant-
Based Management of Dryland Salinity project and the GRDC, started in
2001, has successfully crossed sea barley grass with wheat as part of the
first steps down a long road to breed a more salt-tolerant wheat.
PART III
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TITLE: Developing salt-tolerant crops
Development of a salt-tolerant cereal using 'wide crosses' of
wheat with 'wild' Hordeum species
SOURCE: Cooperative Research Centre for Plant-Based Management of Dryland
Australia
http://www1.crcsalinity.com/pages/project.aspx?projectid=25
DATE: 29 Jul 2004
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Developing salt-tolerant crops
Development of a salt-tolerant cereal using 'wide crosses' of wheat
with 'wild' Hordeum species
One of the objectives of the CRC is to identify and develop new crops that
contribute to improved productivity and profitability of saline lands.
The project aims to develop a salt- and waterlogged-tolerant cereal, and
forms part of the CRC's research into scientifically sound and practical
plant-based approaches to managing salinity in wheat belt areas.
Our aim is to develop cereals capable of extending cropping onto soils
with salinity levels too high for existing cultivars. A number of Hordeum
species inhabit salt marshes and since several Hordeum x wheat hybrids
have been reported, it might be possible to cross these salt tolerant
species with wheat. Hordeum marinum (common sea barley grass) is a species
of particular promise.
This research aims to provide productive options for managing the
increasingly large areas that have and will become salty over the next ten
years.
The research challenge
Sea barley grass (Hordeum marinum) has very high salinity tolerance, and
is capable of growing at levels approaching sea water. It also posssesses
mechanisms for root aeration which contribute to waterlogging tolerance.
The challenge for researchers is to create a successful hybrid with wheat
that maintains these key traits.
Specifically this project will:
1. Identify sources of salt- and waterlogging-tolerance in 'wild' Hordeum
germplasm.
2. Determine which of the salt- and waterlogging-tolerant species can be
crossed with wheat, using cytogenetic techniques
3. Produce cytogenetic stocks (pre-breeding materials) from successful
Hordeum x wheat crosses, and identify lines with a high degree of salt-
and waterlogging- tolerance.
How is the research being done?
The research team is screening 'wild' Hordeum germplasm to find which
species can donate genes for salt- and waterlogging-tolerance, and which
can be hybridised with wheat. The outcomes are uncertain in this cutting-
edge research, because we know little about the 'wild' Hordeum species.
We have:
- produced a Hordeum marinum - wheat amphiploid, demonstrating the
feasibility of using Hordeum marinum in the development of cytogenetical
stocks to transfer traits associated with salt- and waterlogging-tolerance
into bread wheat.
- screened thirty-six Hordeum accessions for waterlogging tolerance in
collaboration with the Swedish University of Agricultural Sciences which
holds the most comprehensive collection of 'wild' Hordeum germplasm in the
world
- screened these same accessions for salinity tolerance in Sweden
- imported accessions of interest to the project and are presently growing
these in a quarantine glasshouse at the University of Adelaide
- collected a diverse range of Hordeum marinum accessions from across the
WA wheat belt
Researchers are now:
- conducting the cytogenetic work to hybridise selected 'wild' Hordeum
species with wheat
- screening these cytogenetic lines for salt- and waterlogging-tolerance
as the lines become available.
Benefits from this research
Salinisation is a threat for up to one-third of agricultural land in
Australia with large areas already salt-affected. Currently the productive
use of saline land is limited to fodder for livestock, with little
opportunity for cropping.
Our objective is to develop a cereal with substaintially more salt
tolerance than current cultivars of barley or wheat, so extending the
range of soils on which cereals can be grown profitability. Not only will
this offcer farmers the benefit of more profitable options for salty land,
it will also give them more flexibility - particularly for those farmers
who do not use livestock in their enterprise.
Salty land is often prone to waterlogged, so waterlogging tolerance is
also needed. Undomesticed or 'wild' species within the Triticeae (same
tribe as wheat and barley) are potential sources of salt- and waterlogging-
tolerance.
Even with these advances, cropping is unlikely to be viable on severely
salt-affected land, where fodders such as saltbush and salt tolerant
grasses will remain the best option.
Nor will salt-tolerant cereals 'solve' the problem of salinity. They might
help use manage some of its symptoms, but will do little to address the
cause of rising water tables, for that we need perennials with high water-
use.
PART IV
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TITLE: DEVELOPING SALT TOLERANT WHEAT
SOURCE: Cooperative Research Centre for Plant-Based Management of Dryland
Australia
http://www1.crcsalinity.com/crc_ar2003/pages/highlights/tolerant_
wheat.html
DATE: 2003
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DEVELOPING SALT TOLERANT WHEAT
Wheat plays an important role in our diets and our export markets, but the
wheat belt of southern Australia contain some of our most threatened
lands. Rising water tables mean that large areas with the highest yield
potential have and continue to become saline and prone to waterlogging. To
maintain crop-based systems substantial improvements in tolerance to these
combined stresses, well above the levels in current cultivars of barley
and wheat, are required.
This is a major challenge for researchers, but promising results in this
part of the landscape could help secure farmer support for more 'radical'
new farming systems in other areas.
The project 'Development of a salt-tolerant cereal using 'wide crosses' of
wheat with 'wild' Hordeum species' aims to develop a salt and waterlogging
tolerant cereal. This ambitious project is lead by Tim Colmer from the
University of Western Australia. It uses 'conventional' breeding
approaches that do not rely on genetic modification.
Undomesticated or 'wild' species within the Triticeae (same tribe as wheat
and barley) are a potential source of salt and waterlogging-tolerance,
particularly the 'wild' Hordeum species that inhabit salt marshes. Sea
barley grass (Hordeum marinum) has very high salinity tolerance, and is
capable of growing at levels approaching sea water. It also possesses
mechanisms for root aeration which contribute to waterlogging tolerance.
The challenge for researchers is to create a successful hybrid that
maintains these key traits.
The most comprehensive collection of 'wild' Hordeum germplasm is held by
the Swedish University of Agricultural Sciences. Collaborative research to
screen for the desired traits and identify key Hordeum species has avoided
delays caused by importing a large number of species through Australian
quarantine processes.
Thirty six Hordeum species (and sub-species) have been screened for
waterlogging tolerance and root aeration traits in Sweden (by Alaina
Garthwaite, a PhD student at UWA with a GRDC-scholarship linked to this
project). The results from these experiments were published in a recent
issue of the peer-reviewed journal 'Functional Plant Biology'. Digby Short
(Research officer appointed to the project) travelled to Sweden in mid-
August 2002 and has been conducting experiments to screen these same
species (and sub-species) for salinity tolerance. The initiative to
conduct these initial screens in collaboration with Professor von Bothmer
in Sweden has enabled quick access to, and preliminary assessment of, a
broad set of representative species in the Swedish Hordeum collection.
Subsequently, 18 accessions of nine species have been imported and grown
(or are presently growing) in a quarantine glasshouse at the Waite Campus,
University of Adelaide.
The results of initial physiological screenings identified H. marinum as
a 'best bet' species, and initial cytogenetical work (by Dr. Rafiq Islam
at Adelaide University) was successful in hybridizing it with bread wheat
(Triticum aestivum). The hybrids were then grown to maturity and treated
with colchicine - a substance which doubles the number of chromosomes to
restore chromosome pairs. The amphiloid cross was then 'back-crossed' with
bread wheat to produce a second generation cross. These plants will then
be screened to find individuals with the extra H. marinum chromosome for
further screening with the ultimate aim of identifying salt and
waterlogging tolerant plants with minimum amounts of chromosome material
from H. marinum in the wheat genetic background.
Early results are promising and the research is progressing to plan. The
production of a H. marinum-wheat cross shows that our approach is
feasible. The research is now only in its second year, and much work
remains.
Ultimately, salt-tolerant wheat is not going to 'solve' the problem of
salinity. While it helps us to manage some of the symptoms, it will do
little to address the cause of rising water tables - for that we need
perennials with high water use. However, it will extend the range of soils
on which wheat can be grown. On severely salinised land cropping is
unlikely to be viable even with salt tolerant wheat, where fodders (such
as saltbush) remain the most appropriate option.
This research is supported by the Grains Research & Development
Corporation (GRDC).
--
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